Effect Of Hesperidin and Rutin On Doxorubicin Induced Testicular Toxicity in Male Rats

The present study aims to investigate whether rutin and hesperidin could protect against acute doxorubicin (DXR)-induced testicular toxicity. Doxorubicin (DXR)– administered rats (25 mg/ kg; three times interaperitoneally/week for two weeks) were pretreated with rutin and/or hesperidin, (50 mg/ kg body weight) three times per week for three weeks and DXR 25mg/kg b.wt three times per week (at the last two weeks of the experiment). Results showed that DXR caused a decrease in free testosterone and FSH levels which were significantly increased as a result of pre-treatment with rutin and/or hesperidin. LH level was increased significantly due to the pretreatment with the mixture of rutin and hesperidin. The pre-treatment of these animals with rutin, hesperidin, and/or both produced a potential increase of the lowered glutathione level in DXR administered control group. Also, glutathione –S-transferase and glutathione peroxidase activities had a significant elevation in all pretreated groups in corresponding to DXR treated group which exhibited a significant decrease as compared to normal control. Testis lipid peroxidation was increased significantly as a result of DXR administration while all the tested agents including rutin, hesperidin or their mixture induced a significant improvement of the elevated values.